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KMID : 0616120160470010055
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Korean Journal of Pharmacognosy
2016 Volume.47 No. 1 p.55 ~ p.61
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Ethyl Acetate Fraction from Petasites japonicus Attenuates Oxidative Stress through Regulation of Nuclear Factor E2-Related Factor-2 Signal Pathway in LLC-PK1 Cells
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Kim Ji-Hyun
Lee Jae-Min
Lee Sang-Hyun
Cho Eun-Ju
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Abstract
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Antioxidant effects and nuclear factor E2-related factor-2 (Nrf-2) signal pathway of methanol extract and 4 fractions [n-hexane, methylene chloride, ethyl acetate (EtOAc), and n-butanol fractions] from Petasites japonicus were investigated. The EtOAc fraction showed highest polyphenol and flavonoid contents among other fractions. In addition, EtOAc fraction showed stronger scavenging activity against superoxide anion radical than other fractions. Furthermore, we investigated antioxidants effects of the EtOAc fraction under cellular system using LLC-PK1 cells. The EtOAc fraction dose-dependently increased the antioxidant protein expressions of heme oxygenase 1 (HO-1) and thioredoxin reductase 1 (TrxR1) known to be involved in oxidative stress, through activation of Nrf-2. The treatment of EtOAc fraction (100 mg/mL) led to the elevation of the high expression of Nrf-2-dependent factor such as HO-1 and TrxR1. These results indicated that the EtOAc fraction of P. japonicus showed high antioxidant activity by regulation of Nrf-2 signaling pathway.
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KEYWORD
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Petasites japonicus , Antioxidant , Heme oxygenase-1 , Nrf-2
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